Amide isomerization pathways II: Tracing geometrical prerequisites of acid-catalyzed amide cis–trans isomerization in globular proteins

Published April 21, 2026 13:18

In this article, with using MD simulations and statistical analysis of PDB structures, we identified favourable orientations of Asp/Glu carboxyl sidechains and water molecules, surrounding cis and trans peptide bonds in protein structures, satisfying the geometric criteria for the key steps of a previously proposed isomerization mechanism.

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