NMR lab.

Facility manager: Dr. Andrea Bodor

Members: Dr. Andrea Bodor, Erika Dudás, Horváth Dániel , Dr. András Láng, Fruzsina Pilhál, Dr. Gyula Pálfy, Fanni Sebák , Csenge Lilla Szabó, Bianka dr. Szalainé Ágoston PhD

In line with modern bioNMR spectroscopy we are dedicated to determine macromolecular structures, especially those of peptides and proteins, elucidate internal dynamics, binding properties, chemical shift mapping, macromolecular interactions, folding and unfolding, etc.. We are providing high quality atomic detail information on polypeptides, miniproteins, protein modules and domains, unstructured proteins (IUP/IDP), etc.. We are helping and providing expertise and disseminate NMR based knowledge to chemists and biochemists, a molecular insight into their current investigation and thus to bring out the most of their research.

Laboratory News

  • Finally out as a VIP article in ChemEurJ

    CoverA fascinating story on the smallest folded protein ever studied: structural insights into the Trp-cage folding intermediate formation. A multi-state folding scenario of a miniprotein at atomic details. By using a novel non-linear fitting method both the thermodynamic parameters and the NMR chemical shifts of the conformers of the multi-state unfolding process is revealed. link

     Figure2 Tc5b Hsqc

Publications

  • Zoltán Gáspári , András Perczel
    Protein dynamics as reported by NMR
    Annu. Rep. NMR Vol. 71, in press (2010)
  • Zoltán Gáspári , Annamária F. Ángyán , Somdutta Dhir , Dino Franklin , András Perczel , Alessandro Pintar , Sándor Pongor
    Probing dynamic protein ensembles with atomic proximity measures
    Curr. Protein Pept. Sci. 11(7):515-522. | DOI: 10.2174/138920310794109201 | PMID: 20887264 (2010) Kivonat
  • Zoltán Gáspári , Péter Várnai , Balázs Szappanos , András Perczel
    Reconciling the lock-and-key and dynamic views of canonical serine protease inhibitor action
    FEBS Lett. 584(1):203-206. | DOI: 10.1016/j.febslet. | PMID: 19932101 (2010) Kivonat
  • Katalin E. Kövér , Tamás Beke , András Lipták , András Perczel
    Combined NMR three-bond scalar coupling measurements and QM calculations to calculate OH-rotamer equilibrium of polyalcohols
    J. Comput. Chem. 30:540-550. (2009) Kivonat
  • Zsuzsa Hódi , Attila L. Németh , László Radnai , Csaba Hetényi , Katalin Schlett , Andrea Bodor , András Perczel , László Nyitray
    Alternatively spliced exon B of myosin Va is essential for binding the tail-associated light chain shared by dynein
    Biochemistry 45:12582-12595. (2006) Kivonat
  • Zotter A , Oláh J , Hlavanda E , Bodor A , Perczel A , Szigeti K , Fidy J , Ovádi J
    Zn(2+)-induced rearrangement of the disordered TPPP/p25 affects its microtubule assembly and GTPase activity.
    BIOCHEMISTRY 50:(44) pp. 9568-9578. (2011) Kivonat
  • Annette Duelli , Bence Kiss , Ida Lundholm , Andrea Bodor , Maxim V. Petoukhov , Dmitri I. Svergun , László Nyitray , Gergely Katona
    The C-terminal random coil region tunes the Ca²⁺-binding affinity of S100A4 through conformational activation
    PLoS One 9(5):e97654 | DOI: 10.1371/journal.pone.0097654 | PMID: 24830809 (2014) Kivonat
  • Andrea Bodor , László Radnai , Csaba Hetényi , Péter Rapali , András Láng , Katalin E. Kövér , András Perczel , Weixiao Y. Wahlgren , Gergely Katona , László Nyitray
    DYNLL2 Dynein Light Chain Binds to an Extended Linear Motif of Myosin 5a Tail That Has Structural Plasticity
    Biochemistry 53(45):7107-7122. | DOI: 10.1021/bi500574z | PMID: 25312846 (2014) Kivonat
  • Andrea Bodor , Katalin E. Kövér , Lena Mäler
    Membrane interactions in small fast-tumbling bicelles as studied by 31P NMR
    BBA Biomembranes 1848(3):760-766. | DOI: 10.1016/j.bbamem.2014.12.001 | PMID: 25497765 (2015) Kivonat
  • Anita Alexa , Gergő Gógl , Gábor Glatz , Ágnes Garai , András Zeke , János Varga , Erika Dudás , Norbert Jeszenői , Andrea Bodor , Csaba Hetényi , Attila Reményi
    Structural assembly of the signaling competent ERK2–RSK1 heterodimeric protein kinase complex
    Proc. Natl. Acad. Sci. USA 112(9):2711-2716. | DOI: 10.1073/pnas.1417571112 | PMID: 25730857 (2015) Kivonat
  • Gergő Gógl , Anita Alexa , Bence Kiss , Gergely Katona , Mihály Kovács , Andrea Bodor , Attila Reményi , László Nyitray
    Structural basis of Ribosomal S6 Kinase 1 (RSK1) inhibition by S100B Protein: modulation of the Extracellular Signal-regulated Kinase (ERK) signaling cascade in a calcium-dependent way
    J. Biol. Chem. 291(1):11-27. DOI: 10.1074/jbc.M115.684928 | PMID: 26527685 (2016) Kivonat
  • Gyula Pálfy , Bence Kiss , László Nyitray , Andrea Bodor
    Multi-level changes in protein dynamics upon complex formation of the calcium-loaded S100A4 with a non-muscle myosin IIA tail fragment
    ChemBioChem 17(19):1829-1838 DOI: 10.1002/cbic.201600280 | PMID: 27418229 (2016) Kivonat