Protein crystallography lab.

Facility manager: Dr. Veronika Harmat

Members: Zsolt Dürvanger, Dániel Forray, Dr. Veronika Harmat, Gergő Hegedűs, Dr. Dóra K. Menyhárd , Anna Kiss-Szemán, Máté Sulyok-Eiler

For a better understanding of function and interactions of biological macromolecules, 3D-structure is required at an atomic resolution, typically determined by protein crystallography; a perfect method of choice. On the other hand, small molecular crystallographic studies explore the structural basis of physical-chemical characteristics of the compound. In 2018. new X-ray crystallographic instruments were installed in the EU 2020 framework (Széchenyi 2020*). The aim of these consortial projects is to establish an infrastructure serving as an open lab for a wider range of research groups (ELTE-CrystalLAB). The Rigaku XtaLAB Synergy-R X-ray diffractometer is capable for small molecular and protein crystallographic data collection as well as in situ crystal testing. A TTP Labtech Mosquito-LCP is available for automated crystallization, and monitoring crystal growth is carried out by a Formulatrix Rock Imager 2 system.

*The research within project No. VEKOP-2.3.3-15-2017-00018 and VEKOP-2-3-2-16-2017-00014 was supported by the European Union and the State of Hungary, co-financed by the European Regional Development Fund.

Laboratory News

  • X-Ray diffraction data collection at synchrotron sources


    We visited European microfocus synchrotron sources at ESRF and BESSY.

    Szink2

  • Scientific meeting at the Hungarian Academy of Sciences on the occasion of the International Year of Crystallography

    We participated at the scientific meeting organised by the Sections of the Physical, Chemical and Biological Sciences of the Hungarian Academy of Sciences on the occasion of the International Year of Crystallography 2014. We presented the story of discovering structure/function relationships of enzymes of the prolyl oligopeptidase family

    Iycr Square 190x182

Publications

  • Dóra K. Menyhárd , Zoltán Orgován , Zoltán Szeltner , Ilona Szamosi , Veronika Harmat
    Catalytically distinct states captured in a crystal lattice: the substrate-bound and scavenger states of acylaminoacyl peptidase and their implications for functionality
    Acta Crystallogr D Biol Crystallogr. 71(Pt 3):461-472. | DOI: 10.1107/S1399004714026819 | PMID: 25760596 (2015) Kivonat
  • Veronika Harmat , Klarissza Domokos , Dóra K. Menyhárd , Anna Palló , Zoltán Szeltner , Ilona Szamosi , Tamás Beke , Gábor Náray-Szabó , László Polgár
    Structure and catalysis of acylamonoacyl peptidase: closed and open subunits of a dimer oligopeptidase
    Journal of Biological Chemistry 286:1987-1998. PMID: 21084296 | DOI: 10.1074/jbc.M110.169862 (2011) Kivonat
  • Dóra K. Menyhárd , Anna Kiss-Szemán , Éva Tichy-Rács , Balázs Hornung , Krisztina Rádi , Zoltán Szeltner , Klarissza Domokos , Ilona Szamosi , Gábor Náray-Szabó , László Polgár , Veronika Harmat
    A self-compartmentalizing hexamer serine protease from Pyrococcus horikoshii: substrate selection achieved through multimerization
    J Biol Chem 288(24):17884-17894. | DOI: 10.1074/jbc.M113.451534 | PMID: 23632025 (2013) Kivonat
  • Anna J. Kiss-Szemán , Veronika Harmat , Dóra K. Menyhárd
    Achieving Functionality Through Modular Build-up: Structure and Size Selection of Serine Oligopeptidases
    Current Protein & Peptide Science 1089 - 1101 DOI:10.2174/1389203720666190925103339 (2019) Kivonat
  • Valéria Bugris , Veronika Harmat , Györgyi Ferenc , Sándor Brockhauser , Ian Carmichael , Elspeth F. Garman
    Radiation-damage investigation of a DNA 16-mer
    Journal of Synchrotron Radiation 998-1009 doi.org/10.1107/S160057751900763X (2019) Kivonat
  • Zsolt Dürvanger , Veronika Harmat
    Structural Diversity in Calmodulin - Peptide Interactions
    Current Protein & Peptide Science 1102 - 1111 doi.org/10.2174/1389203720666190925101937 (2019) Kivonat
  • József Dobó , Veronika Harmat , László Beinrohr , Edina Sebestyén , Péter Závodszky , Péter Gál
    MASP-1, a Promiscuous Complement Protease: Structure of Its Catalytic Region Reveals the Basis of Its Broad Specificity
    J. Immunol. 183:1207-1214. (2009) Kivonat
  • László Beinrohr , Veronika Harmat , József Dobó , Zsolt Lörincz , Péter Gál , Péter Závodszky
    C1 inhibitor serpin domain structure reveals the likely mechanism of heparin potentiation and conformational disease
    J. Biol. Chem. 282: 21100-21109. (2007) Kivonat
  • Márton Megyeri , Veronika Harmat , Balázs Major , Ádám Végh , Júlia Balczer , Dávid Héja , Katalin Szilágyi , Dániel Datz , Gábor Pál , Péter Závodszky , Péter Gál , József Dobó
    Quantitative characterization of the activation steps of Mannan-Binding Lectin (MBL)-Associated Serine Proteases (MASPs) points to the central role of MASP-1 in the initiation of the complement lectin pathway
    J Biol Chem 288(13):8922-8934. | DOI: 10.1074/jbc.M112.446500 | PMID: 23386610 (2013) Kivonat
  • Viktória Goldschmidt Gőz , István Pintér , Veronika Harmat , András Perczel
    Approaches to Pyranuronic beta-Sugar Amino Acid Building Blocks of Peptidosaccharide Foldamers
    Eur. J. Org. Chem. 355-361. DOI: 10.1002/ejoc.201701612 (2018) Kivonat
  • Tibor Pasinszki , Dániel Dzsotján , Győző György Lajgut , Veronika Harmat , Antal Csámpai , Andrea Bor , István Zupkó
    Synthesis, spectral- and theoretical study, x-ray analysis, and antiproliferative activity of 4,5-dihydrobenzoferroceno[1,2-d][1,2,3]selenadiazole and its benzo-fused analogue
    Journal of Organometallic Chemistry 863:70-76. | DOI: 10.1016/j.jorganchem.2018.03.031 (2018) Kivonat
  • Roland Szalay , Veronika Harmat , János Eőri , Gábor Pongor
    Strong influence of intramolecular Si⋯O proximity on reactivity: Systematic molecular structure, solvolysis, and mechanistic study of cyclic N-trimethylsilyl carboxamide derivatives
    Tetrahedron Letters 58(23):2186-2192. | DOI: 10.1016/j.tetlet.2017.04.057 (2017) Kivonat
  • Anikó Angyal , András Demjén , Veronika Harmat , János Wölfling , László G. Puskás , Iván Kanizsai
    1,3-Dipolar Cycloaddition of Isatin-Derived Azomethine Ylides with 2H‐Azirines: Stereoselective Synthesis of 1,3- Diazaspiro[bicyclo[3.1.0]hexane]oxindoles
    The Journal of Organic Chemistry 4273-4281 DOI: 10.1021/acs.joc.9b00242 (2019) Kivonat
  • Kinga Judit Fodor , Dániel Hutai , Tamás Jernei , Angéla Takács , Zsófia Szász , Máté Sulyok-Eiler , Veronika Harmat , Rita Oláh Szabó , Gitta Schlosser , Ferenc Hudecz , László Kőhidai , Antal Csámpai
    Novel Polycondensed Partly Saturated β-Carbolines Including Ferrocene Derivatives: Synthesis, DFT-Supported Structural Analysis, Mechanism of Some Diastereoselective Transformations and a Preliminary Study of their In Vitro Antiproliferative Effects
    Molecules 25(7), 1599 | doi: 10.3390/molecules25071599 (2020) Kivonat